ABSTRACT
Objective To evaluate the effect and mechanism of cysteine rich protein 61, namely CCN family member 1(CCN1) on the survival of adipose tissues in rats after autologous fat grafting. Methods At 1 week after the establishment of autologous fat grafting rat models, all animals were randomly divided into the CCN1 group (n=20) and control group (n=20). The survival of fat grafts, the morphology of fat graft tissues, the proportion of active adipocytes and the number of new blood vessels of rats were statistically compared between two groups. The levels of differential expressed messenger ribonucleic acid (mRNA) in the fat graft tissues of rats were compared between two groups by high-throughput sequencing and subsequently subject to cluster analysis. The expression levels of related proinflammatory cytokines of fat graft tissues of rats were statistically compared between two groups. Results The weight retention rate of adipose tissues in the CCN1 group was significantly higher than that in the control group (P < 0.05). In the CCN1 group, the integrity of adipocytes was considerably higher, the degree of vesiculation and vacuolation, the degree of inflammatory cell aggregation and the degree of fibrosis were significantly lower than those in the control group (all P < 0.000 1). Immunofluorescence staining demonstrated that the proportion of active adipocytes with uniform morphology was higher in the CCN1 group, whereas the proportion of active adipocytes was lower and the cells were observed in different sizes accompanied by vesiculation in the control group. Compared with the control group, the quantity of new blood vessels was significantly higher, and the expression levels of platelet derived growth factor (PDGF) and fibroblast growth factor (FGF) mRNA were remarkably higher in the CCN1 group (all P < 0.05). High-throughput sequencing analysis showed that the data at the transcriptome levels significantly differed between two groups. In the CCN1 group, the gene expression levels of cell surface markers, inflammatory cytokines and chemokines related to M1 macrophages tended to decline. Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) revealed that the mRNA expression levels of interleukin (IL)-8, IL-1 and Toll-like receptor (TLR) 2 in the CCN1 group were significantly lower than those in the control group (P < 0.01-0.05). Conclusions During autologous fat grafting, supplement of exogenous CCN1 may effectively promote the neovascularization of adipose tissues and improve the survival rate of fat graft probably by mediating the transformation of macrophages into M2 phenotype via down-regulating the TLR2 expression level.
ABSTRACT
Objective@#This study aims to determine the prevalence of overweight and obesity and associated early life factors among children aged 2 to 6 years in a community in Guangzhou.@*Methods@#A stratified cluster sampling was used to select 922 children from one community in Guangzhou in 2018. The survey included a questionnaire survey and physical examination. Logistic regression analyses were performed to examine the relationship of early-life factors and obesity.@*Results@#The prevalence of overweight and obesity was 13.4% in 922 children(16.1% in boys and 10.1% in girls). The sex difference in prevalence of overweight and obesity was statistically significant(Z=2.69, P<0.05). Overweight and obesity was found significantly higher in children with large for gestational age, caesarean section, artificial feeding within 6 months, maternal pre-pregnancy overweight and obesity, maternal excessive weight gain(P<0.01). Large for gestational age (OR=2.62, 95%CI=1.42-4.82), caesarean section(OR=1.59, 95%CI=1.08-2.36), artificial feeding within 6 months(OR=2.00, 95%CI=1.19-3.37), maternal pre-pregnancy overweight or obesity(OR=1.97, 95%CI=1.08-3.58), and excessive gestational weight gain (OR=2.07, 95%CI=1.26-3.39) was positively associated with childhood overweight obesity(P<0.05). Maternal pre-pregnancy underweight was negatively associated with childhood overweight obesity(OR=0.51, 95%CI=0.29-0.88, P<0.05).@*Conclusion@#Early-life risk factors are positively associated with the risk of overweight and obesity in later childhood, including large gestational age, caesarean section, artificial feeding within 6 months, maternal pre-pregnancy overweight and excessive gestational weight gain, while maternal pre-pregnancy underweight associates with low risk of child overweight and obesity. These findings suggest that early intervention to these modifiable risk factors could make a significant contribution to childhood obesity prevention.